Mouse anti-4-amino Biphenyl DNA monoclonal antibody for IHC, ICC, IF, ELISA. 4-amino Biphenyl (4-ABP) is an aromatic amine (arylamine). 4-ABP can be found as a constituent of tobacco smoke, in the form of an environmental carcinogen. It is metabolized by hepatic cytochrome P4501A2 to yield a direct-acting mutagen known as N-hydroxy-aminobiphenyl. Yet, the accumulation of biologically active metabolites is dependent on the activation and detoxification of N-acetylation. 4-ABP has been shown to be a bladder carcinogen in humans and rodents. As well as genetic factors, 4-ABP may also play a role in breast cancer. It is hypothesized that smokers may have higher levels of DNA adducts than non-smokers and that the role of 4-ABP-DNA adducts and their relationship to tobacco exposure may be related to breast tumorigenesis. The antibody has been used to investigate the role of 4-ABP in the study of human breast cancer by measuring 4-ABP-DNA adducts.Cellular Localization: NuclearApplications: Suitable for use in Immunofluorescence, ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution:ELISA: 1:50-1:100Immunohistochemistry: 1:50-1:100Immunocytochemistry: 1:50-1:100Immunofluorescence: 1:50-1:100Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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