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Anti-ACTB (N-terminal arginylated ) polyclonal antibody

Cat no: CABT-B1633

Anti-ACTB (N-terminal arginylated ) polyclonal antibody

Rabbit Anti-Human ACTB polyclonal antibody for WB, Dot, ICC. Actin, cytoplasmic 1 (UniProt P60709; also known as beta-actin, PS1TP5-binding protein 1) is encoded by the ACTB (also known as BRWS1, PS1TP5BP1) gene (Gene ID 60) in human. Post-translational protein arginylation is mediated by Arg–transfer RNA (-tRNA) protein transferase (Ate1; arginyltransferase). ?- and ?-actins are the major structural components of the actin cytoskeleton in nonmuscle cells. Despite their similarity, the ?-, but not the ?-, actin is N-terminally arginylated in vivo, where the first two N-terminal amino acids are removed and replaced with an arginine (from NH2-MDDDIAAL- to NH2-RDDIAAL-). In the absence of arginylation, actin forms aggregates during cell-free polymerization. Fibroblasts derived from Ate1 knockout (KO) mice show collapsed lamella, which can be rescued by reintroducing N-terminally arginylated ?-actin into the Ate1 KO cells. Intracellular actin polymer levels are shown to drastically decrease in arginylation-deficient cells, which results from changes in actin polymerization properties.

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SPECIFICATIONS

Catalog Number

CABT-B1633

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Target

ACTB

Gene Id

60

Clonality

Polyclonal

Storage Temp

Stable for 1 year at 2-8В°C from date of receipt.

SUPPLIER INFO

Creative Diagnostics

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