anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody

anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody: Ki-67 is a nuclear protein which is expressed during every cell cycle except in G0. Anti-Ki-67 antibody allows classifying which cells are proliferating or are stationary. So it is possible to study the growing fraction or the mitotic rate in a cell population. It is particularly useful in malign tumors studies and others pathological states quantifying the proliferating cells proportion. Ki-67 shows a highly specificity for proliferating cells than PCNA (Proliferating Cells Nuclear Antigen), but it has a shorter half-life. Therefore, proliferating cells detection by using Ki-67 is the most widely used himmunohistochemical method. Anti-Ki-67 antibody stains lipoblasts and other cells which contains fats, so it can also be used for the liposarcomas detection. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.

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