Anti-BIG2 polyclonal antibody

Rabbit Anti-Rat BIG2 polyclonal antibody for WB, ICC. Mammalian BIG2 (BFA-inhibited guanine nucleotide-exchange protein 2) is encoded by the Arfgef2 gene in rat (NCBI Gene ID 296380). The fungal antibiotic Brefeldin A causes disassembly of the Golgi complex and ER swelling in mammalian cells by inhibiting three ADP-ribosylation factor (ARF) guanine nucleotide-exchange factors (GEFs), BIG1, BIG2, and GBF1, all of which interact with various ARFs via their respective Sec7 domains. ARF4/5 activation by GBF1 is shown to facilitate BIG1/2 recruitment to the trans-Golgi network (TGN) and endosomes, where BIG1/2 mediate ARF1/3 activation and lead to recruitment of clathrin adaptors. Independent of its Sec7 domain-mediated GEF activity, BIG2 is also known to interact with other cellular proteins and function as a scaffold for assembling multimolecular complexes involved in several cellular processes.

Prices direct from Creative Diagnostics

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

CABT-B1557

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Target

ARFGEF2

Gene Id

296380

Clonality

Polyclonal

Storage Temp

Stable for 1 year at -20В°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20В°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.