Mouse anti-Calcifediol monoclonal antibody for ELISA. Suitable for use in ELISA (1:10,000). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.Vitamin D is a groupof fat-soluble secosteroids, the two major physiologically relevant forms ofwhich are vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol).Vitamin D without a subscript refers to either D2 or D3 or both. Vitamin D3is produced in the skin of vertebrates after exposure to ultraviolet B lightfrom the sun or artificial sources, and occurs naturally in fish and a fewother foods. In some countries, staple foods such as milk, flour andmargarine are artificially fortified with vitamin D, and it is also availableas a supplement in pill form. Light-exposed mushrooms may provide up to 100%of the recommended Daily Value of vitamin D