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Anti-Cas9 monoclonal antibody, clone 8B10-4B4

Cat no: DCAB-WB151

Anti-Cas9 monoclonal antibody, clone 8B10-4B4

Mouse anti-Cas9 monoclonal antibody for WB; IHC. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

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SPECIFICATIONS

Catalog Number

DCAB-WB151

Hosts

Mouse

Conjugates

Unconj

Clone

8B10-4B4

Target

CRISPR-Cas9

Isotype

IgG1

Clonality

Monoclonal

Storage Temp

Shipped at 4В°C. Store at +4В°C short term (1-2 weeks). Store at -20В°C long term.

SUPPLIER INFO

Creative Diagnostics

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