anti-CD14 Molecule (CD14) antibody: CD14 is a 55 kDa protein working as a receptor which binds the complex of lipopolysaccharids (LPS) and LPSbinding protein (LBP). It is mainly expressed on the surface of monocytes and macrophages. The staining of CD14 is especially helpful for the detection of normal and neoplastic cells of the monocytic cell lineage. This anti-CD14 antibody stains monocytes/macrophages and Langerhans cells in Langerhans cell histiocytosis as well as tumour cells in monocytic leukaemia and histiocytic lymphomas. Additionally, the antibody reacts with diffuse large B-cell lymphomas and splenic marginal zone lymphoma but not with other B-cell lymphomas. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
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