Mouse anti-FLAG tag monoclonal antibody for WB, IP, ICC, IF, FC. FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. The peptide sequence of the FLAG-tag is: N-DYKDDDDK-C (1012Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies recognize the epitope only when it is present at the N-terminal. However, other available antibodies are position-insensitive.
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