Mouse anti-FLAG tag monoclonal antibody for WB, IP, IS. The peptide sequence of the FLAG-tag is: N-DYKDDDDK-C (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminal. However, other available antibodies (e.g., M2) are position-insensitive. The FLAG-tag was the first example of a fully functional epitope tag to be published in the scientific literature and, being the first, was the only epitope tag to be patented. Unlike some other tags (e.g. myc, HA), where a monoclonal was first isolated against an existing protein, then the epitope was characterized and used as a tag, the FLAG epitope was designed first, and then monoclonals were raised to recognize it. The FLAG tag"s structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. In addition, N-terminal FLAG tags can be removed readily from proteins once they have been isolated, by treatment with the specific protease, enterokinase.
Quick Search
Products 
