Anti-GFP tag antibody, clone HGQ. D3

Mouse anti-GFP tag monoclonal antibody for ELISA, Dot, WB, IP, IS. Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

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SPECIFICATIONS

Catalog Number

DMAB8894

Applications

ELISA

Hosts

Mouse

Conjugates

Unconj

Clone

HGQ. D3

Target

GFP tag

Isotype

IgG1

Clonality

Monoclonal

Storage Temp

Stable for 1 year at -20В°C and 3 months at 4В°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.