anti-H-2Dd antibody (Biotin): The antibody detects: A conformational epitope on the 1 and 2 chains of mouse H-2Dd class I alloantigen MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of 1 and 2 chains when complexed with 2-microglobulin.2 The antibody does not react with H-2Dd chains synthesized in vitro.3 Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis.4 Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed.2 MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.