Anti-Hepatitis B Virus E Antigen (HBeAg) ELISA Kit, BioAssay(TM)

Principle of the Assay:\nThe purified Anti-HBe is coated on the solid phase of multi-wells. Serum sample, recombinant HBeAg and horseradish peroxidase labeled with Anti-HBe (conjugated) are added to coated wells, and form a competitive combination. After incubation, if Anti-HBe content is high in the sample, a complex of HBeAg-Anti-HBe-Anti-HBe labeled with HRP will form. The wells are washed to remove the complex, and incubated with substrate (TMB), subsequently producing no color change or little color change. If Anti-HBe is not present in the sample, there will be significant color change. The test is sensitive, reproducible and easy to operate.\n\nThe Anti-Hepatitis B Virus E Antigen (HBeAg) ELISA Kit is a qualitative in vitro enzyme immunoassay for the detection of anti-HBe in human serum or plasma.\n\nKit Components:\nH1910-40J1: Microtiter Plate, 1x96wells\nH1910-40J2: Enzyme Conjugate, 1x6ml\nH1910-40J3: Positive Control Serum, 1x500ul\nH1910-40J4: Negative Control Serum, 1x500ul\nH1910-40J5: Wash Buffer (20X), 1x30ml\nH1910-40J6: Substrate A, 1x6ml\nH1910-40J7: Substrate B, 1x6ml\nH1910-40J8: Stop Solution, 1x6ml\nH1910-40J9: Neutralization Reagent, 1x6ml\n\nInterpretation of Results:\nCut-off Value (COV) Calculation:\n\nCOV = average OD450 of negative controls X 0.5\n\nPositive: OD450 of sample < COV\nNegative: OD450 of sample (same/more than) COV\nInvalid: If the OD of negative control is below or equal to 1.000, the result is invalid. In any event, repeat the test. If the problem persists, contact technical support.\n\nPerformance Characteristics:\nSensitivity: 4NCU/ml (per Biological Reference Reagents of Chinese Clinical Test Center), OD (same/less than) 0.600\nSpecificity: The average OD of 20 normal negative smples is (same/more than) 2.00\nPrecision: %CV (same/less than) 20% (n= 10)\n\nStorage and Stability:\nStore all kit components at 4C. Kit is stable for 6 months.

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