Catalogue number: | ABIN361731 |
Reactivities: | Human, Rat |
Hosts: | Mouse |
Applications: | Immunoprecipitation |
Size: | 50 micro g |
Clone: | 8D3 |
Concentration: | 1 mg/mL |
Antigen: | Hsp90 Complex |
Isotype: | IgM |
Clonality: | Monoclonal |
Sensitivity: | Goat anti-mouse IgM was used to bind 25 myl of protein G-Sepharose. SMC-109 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 myl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including hsp90, hsp70 and Hop. |