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Anti-Lysenin polyclonal antibody

Cat no: DPAB21490H

Anti-Lysenin polyclonal antibody

Rabbit anti-Lysenin polyclonal antibody. Lysenin, a novel 41-kDa protein purified from coelomic fluid of the earthworm Eisenia foetida, induced erythrocyte lysis. Preincubation of lysenin with vesicles containing sphingomyelin inhibited lysenin-induced hemolysis completely, whereas vesicles containing phospholipids other than sphingomyelin showed no inhibitory activity, suggesting that lysenin bound specifically to sphingomyelin on erythrocyte membranes. The specific binding of lysenin to sphingomyelin was confirmed by enzyme-linked immunosorbent assay, TLC immunostaining, and liposome lysis assay. In these assays, lysenin bound specifically to sphingomyelin and did not show any cross-reaction with other phospholipids including sphingomyelin analogs such as sphingosine, ceramide, and sphingosylphosphorylcholine, indicating that it recognized a precise molecular structure of sphingomyelin.

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SPECIFICATIONS

Catalog Number

DPAB21490H

Hosts

Rabbit

Conjugates

Unconj

Target

Lysenin

Clonality

Polyclonal

Storage Temp

-20 В°C, Avoid freeze / thaw cycles

SUPPLIER INFO

Creative Diagnostics

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