anti-Microphthalmia-Associated Transcription Factor (MITF) antibody

anti-Microphthalmia-Associated Transcription Factor (MITF) antibody: MiTF (Microphthalmia associated Transcription Factor) is a helix-loop-helix transcription factor implicated in pigmentation and mast and bone development. MiTF gene mutation produces Waardenburg IIA and the Tietze syndrome, they causes pigmentation and deafness disorders, by melanocytes deficiencies in inner ear. There are two known isoforms of the MiTF gene which differ in 66 amino acids in N-terminus. The shorter MiTF isoform (it runs two bands, 52 and 56 kDa) specific of melanocytes is MiTF, which expresses either in normal or malignant melanocytes. The other forms MiTF-A, MiTF-C and MiTF-H runs a bands cluster between 60-70 kDa, they differ structurally of MiTF in the N- terminus, they are expressed in osteoclasts and mast cells and heart, and the B16 melanoma line cell (but not in other line cells). MiTF plays an important role in several cell type differentiation. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.

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