Goat anti-Mouse IgG1 polyclonal antibody for ELISA, WB.. Suitable for use in ELISA (coating 1:200 –1:500) and Western blot (1:5,000–1:15,000). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).