Anti-Mullerian Hormone (AMH, Muellerian-inhibiting Factor, Muellerian-inhibiting Substance, MIS)

Anti-mullerian hormone (AMH) is originally classified as a foetal testicular hormone that inhibits Mullerian duct development. AMH is expressed post-natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth. AMH is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer.\n\nApplications:\nSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:20-1:40 Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer, pH 6.0 is recommended.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control Tissue:\nOvary\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from T/O outbred mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2298-11M

Size

1ml

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, NHP, Sheep

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Van Saen, D. et al. (2010) Meiotic activity in orthotopic xenografts derived from human postpubertal testicular tissue.Hum Reprod. Nov 30. [Epub ahead of print] 2. Gruijters, M. et al. (2003) Mol. Cell. Endocrinol. 211: 85-90. 3. Weenen, C. et al. (2004) Mol. Hum. Reprod. 10(2):77-83. 4. Papanastasopoulos, P. et al. (2009) AFertil Steril. 92: 1169.e11-4. 5. Campbell, B.K. (2009) The endocrine and local control of ovarian follicle development in the ewe Anim. Reprod. 6:159-71 6. Walker, M.L. et al. (2009) Reproduction. 138: 793-9.7. Sobinoff, A.P. et al. (2011) Understanding the villain: DMBA induced pre-antral ovotoxicity involves selective follicular destruction and primordial follicle activation through PI3K/Akt and mTOR signalling.Toxicol Sci. Jul 23. [Epub ahead of print]8. Van Saen, D. et al. (2011) Can pubertal boys with Klinefelter syndrome benefit from spermatogonial stem cell banking.Hum Reprod. Dec 12. [Epub ahead of print]

Additional Info

Recognizes human Anti-Mullerian Hormone. Species Crossreactivity: mouse, sheep, monkey