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Anti-Nuclear Antibodies, Human (ANA) BioAssay(TM) ELISA Kit

Cat no: A2298-12K

Anti-Nuclear Antibodies, Human (ANA) BioAssay(TM) ELISA Kit

The United States Biological ANA Screen ELISA test system is designed to detect IgG class antibodies to a variety of common nuclear antigens in human sera. Wells of plastic microwell strips are sensitized by passive absorption with antigen. \n\nKit Components: \nAll reactive reagents contain sodium azide as a preservative at a concentration of 0.1%. \nA2298-12K1: Microtiter Plate, 1x96 wells, coated with capture antibody. \nA2298-12K2: Conjugate, IgG (HRP) Gt x Hu, lambda chain specific, 1x15ml \nA2298-12K3: Calibrator (Human Serum) CF=0.41, 1x500ul\nA2298-12K4: Positive Control (Human Serum) 1x350ul\nA2298-12K5: Negative Control (Human Serum) 1x350ull\nA2298-12K6: Sample Diluent, 1x30ml (Contains PBS, pH7.2, BSA, Tween-20)\nA2298-12K7: Wash Buffer, 10X, 1x100ml, (Contains PBS, Tween-20) \nA2298-12K8: Tetramethylbenzidine (TMB), 1x15ml (Contains <15% DMSO) \nA2298-12K9: Stop Solution, 1x15ml (1M H2SO4, 0.7M HCl) \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months, For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2298-12K

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Tan E, et al., Arthritis Rheum. 25:1271-1277 (1982). 2. Beufels M, et al., Am. J. Med. 74:201-215 (1983). 3. Sharp GC, et al., Am. J. Med. 52: 148-159 (1972). 4. Winfield JB, et al., Arthritis Rheum. 21:289-294 (1978). 5. Tan EM, Kunkel HG., J. Immunol. 96:464-471 (1966). 6. Maddison PJ, et al., J. Rheumatol. 6:189-192 (1979). 7. Clark G, et al., J. Immunol. 102:117 (1969). 8. Alexander E, A. et al., J. Rheum. 9:239-246 (1982). 9. Alspaugh MA, et al., Arthritis Rheum. 19:216-222 (1976). 10. Marguerie C, et al., Quart. J. Med. 77:1019-1038 (1990). 11. Tan EM., Adv. Immunol. 44:93-151 (1989). 12. Sontheimer RD, et al., Arch. Derm. 115:1409-1415 (1979). 13. Provost, T.T., et al., Arth. Rheum. In Press. 14. LeRoy EC, et al., J. Rheumatol. 15:202-205 (1988). 15. Weiner ES, et al., . Arthritis Rheum. 34:68-77 (1991). 16. Mongey AB, et al., Advances in Int. Med. 36: 151-169(1989). 17. Procedures for the Handling and Processing of Blood Specimens. NCCLS Document H18-A, 10:12, Approved Guideline (1990). 18. Procedures for the collection of diagnostic blood specimens by venipuncture. 2nd edition. Approved Standard (1984). Published by National Committee for clinical Laboratory Standards.19. Sturgess A: Review; Recently characterized autoantibodies and their clinical significance. Aust. N.Z., J. Med. 22:279-289 (1992).

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