Rabbit Anti-Human RBPJ polyclonal antibody for WB, ChIP, ChIPseq. Recombining binding protein suppressor of hairless (UniProt Q06330; also known as CBF-1, csI, H-2K binding factor-2, Immunoglobulin kappa J region recombination signal binding protein 1, J kappa-recombination signal-binding protein, RBP-J, RBP-JK, RBP-J kappa, Renal carcinoma antigen NY-REN-30, Suppressor of hairless homolog) is encoded by the RBPJ (also known as AOS3, CBF1, IGKJRB, IGKJRB1, KBF2, RBPJK, RBPSUH, SUH) gene (Gene ID 3516) in human. RBPJ/RBP-JK is a DNA-binding protein that functions as a transcriptional switch and plays an important role in regulating Notch signaling. RBPJ associates with several transcriptional co-repressors in the absence of Notch intracellular domain (NICD). Upon activation by gamma-secretase cleavage, NICD translocates to the nucleus and forms a transcriptional activation complex with RBPJ. This complex in turn recruits other coactivators and complex components, leading to regulated target gene expression. Genome-wide ChIP-seq profiling reveals both overlapping and non-overlapping chromatin-binding sites occupied by RBPJ and Notch1. Murine RBPJ-only sites are shown to be highly enriched for imputed REST (a DNA-binding transcriptional repressor) sites, whereas human RPBJ-only sites lack REST motifs and are more enriched for imputed CREB sites. Dysregulated RBPJ-dependent gene expression is seen in B-cell tumors associated with Epstein-Barr virus (EBV) and a majority of T-lymphoblastic leukemia/lymphoma (TLL) tumors having somatic Notch1 gain-of-function mutations.
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