Anti-RFP tag antibody, clone SGQ. L9

Mouse anti-RFP tag monoclonal antibody for ELISA, Dot, WB, IP, IS. Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.

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SPECIFICATIONS

Catalog Number

DMAB8888

Applications

ELISA

Hosts

Mouse

Conjugates

Unconj

Clone

SGQ. L9

Target

RFP tag

Isotype

IgG1

Clonality

Monoclonal

Storage Temp

Stable for 1 year at -20В°C and 3 months at 4В°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.