Rabbit anti-Human RNF7 polyclonal antibody for ELISA, WB, IP. Suitable for use in ELISA (1:2,000 – 1:10,000) and Western blot (1:500 – 1:1,000). The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3 and others). Coimmunoprecipitation of related cullin proteins does occur. Coimmunoprecipitation of ubiquitin ligase activity will also occur. A 12.6kDa band corresponding to human ROC2 is detected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.