Anti-TFEB (phospho S142) polyclonal antibody

Rabbit Anti-Human TFEB polyclonal antibody for WB. Transcription factor EB (UniProt P19484; also known as bHLHe35, Class E basic helix-loop-helix protein 35) is encoded by the TFEB (also known as ALPHATFEB, BHLHE35, TCFEB) gene (Gene ID 7942) in human. TFEB is a basic Helix-Loop-Helix (bHLH) leucine zipper transcription factor that plays a major role in the regulation of lysosomal biogenesis. TFEB is also a main player in the transcriptional response to starvation and controls autophagy by positively regulating autophagosome formation and autophagosome-lysosome fusion. TFEB is phosphorylated by mTORC1 and ERK at Ser142, which prevents its nuclear translocation. Pharmacological inhibition of mTORC1, as well as starvation and lysosomal disruption, activates TFEB nuclear translocation. Similarly, the serine-threonine kinase RIP1/RIPK1 is shown to suppress basal autophagic flux by activating ERK, which in turn phosphorylates TFEB at Ser142 and thereby prevents TFEB-mediated transcription of gene products involved in autophagy.

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SPECIFICATIONS

Catalog Number

CABT-B1749

Hosts

Rabbit

Reactivities

Hum

Target

TFEB

Gene Id

7942

Clonality

Polyclonal

Storage Temp

Stable for 1 year at -20В°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20В°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20В°C may cause glycerol containing solutions to become frozen during storage.