Mouse anti-Vimentin monoclonal antibody for WB, ELISA, IF, IHC, ICC. Suitable for use in Western blot, ELISA, immunofluorescence microscopy and immunocyto/histochemistry (frozen and paraffin-embedded). With paraffin-embedded sections, protease pretreatment is required prior to antibody application. Dilute at least 1:10 with PBS, pH 7.4 for immunohistochemical applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.Vimentinsare class-III intermediate filaments found in various non-epithelial cells,especially mesenchymal cells. Vimentin is a type IIIintermediate filament (IF) protein that is expressed in mesenchymal cells. IFproteins are found in all metazoan cellsas well as bacteria. IF, along withtubulin-based microtubules and actin-based microfilaments, comprise thecytoskeleton. All IF proteins are expressed in a highlydevelopmentally-regulated fashion; vimentin is the major cytoskeletalcomponent of mesenchymal cells. Because of this, vimentin is often used as amarker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymaltransition (EMT) during both normal development and metastatic progression.
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