Antioxidant Capacity, Total BioAssay(TM) Kit

Antioxidants play an important role in preventing the formation of and scavenging of free radicals\nand other potentially toxic oxidizing species. There are three categories of antioxidant species:\nenzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). Different antioxidants vary in their reducing power. Trolox is used to standardize antioxidants, with all other antioxidants being\nmeasured in Trolox equivalents. Measurement of the combined nonenzymatic antioxidant capacity of biological fluids and other samples provides an indication of the overall capability to counteract reactive oxygen species (ROS), resist oxidative damage and combat oxidative stress?related diseases. In some cases, the antioxidant contribution of proteins is desired whereas in other cases only the contribution of the small molecule antioxidants is needed. The TAC Assay Kit can measure either the combination of both small molecule antioxidants and proteins or small molecules alone in the presence of proprietary Protein Mask. Cu++ ion is converted to Cu+ by both small molecule and protein. The Protein Mask prevents Cu++ reduction by protein, enabling the analysis of only the small molecule antioxidants. The reduced Cu+ ion is chelated with a colorimetric probe giving a broad absorbance peak around 570nm, proportional to the total antioxidant capacity.\n\nKit Components:\nA2298-43A: Cu2+ Reagent, 1x200ul. Ready to use as supplied.\nA2298-43B: Assay Diluent, 1x10ml. Ready to use as supplied.\nA2298-43C: Protein Mask, 1x10ml. Ready to use as supplied.\nA2298-43D: Trolox Standard, 1x1vial (1x1umole). Supplied as a lyophilized powder. Dissolve in 20ul of pure DMSO by vortexing, then add 980ul of ddH2O and mix well, generating a 1mM solution. Aliquot and store at -20. Aliquots are stable for 4 months at -20C.\n\nStorage and Stability:\nStore all kit components except *A2298-43D at 4 degrees C. Stable for 6 months. Store *A2298-43D at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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