Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Peptide ELISA: 1:32,000.
Western Blot: 0.5-1.5ug/ml. A Band is detected at ~35kD band observed in Human lysates of cell lines A431 and K562 (calculated MW of 38.7kD according to NP_000691.1). Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can
Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See A2295-15N-P. Antibody