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AP-2 alpha

Cat no: A2298-59D

AP-2 alpha

The sequence-specific transcription factor activator protein 2 alpha (AP-2 alpha) is required for normal growth and morphogenesis during mammalian development (1,2). Decreased or loss of AP-2 alpha expression has been observed in many different types of human cancers including breast cancer (3,4), ovarian cancer (5), melanoma (6) and prostate cancer (7). These findings suggest that AP-2alpha expression plays a crucial role in tumorigenicity. Studies have also shown that p53 overexpression in human breast carcinoma cells induces the level of AP-2 alpha expression. Furthermore, p53 binds to the cis-element in the AP-2alpha promoter, suggesting that AP-2 alpha is a target of p53. AP-2 alpha may mediate the effect of p53 to inhibit cell proliferation (8).\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern blot: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:300\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2298-59D

Size

100ul

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by protein A and peptide affinity chromatography

References

1. Mitchell, P.J. et al. (1987) Cell 50; 847

Additional Info

Detects endogenous levels of total human AP-2 alpha protein. Species Crossreactivity: mouse, rat and monkey.

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