Home  >  Products  >  AP1g1 (AP-1 Complex Subunit gamma-1, Adapter-related Protein Complex 1 Subunit gamma-1, Golgi Adaptor HA1/AP1 Adaptin Subunit gamma-1, Clathrin Assembly Protein Complex 1 gamma-1 Large Chain)
AP1g1 (AP-1 Complex Subunit gamma-1, Adapter-related Protein Complex 1 Subunit gamma-1, Golgi Adaptor HA1/AP1 Adaptin Subunit gamma-1, Clathrin Assembly Protein Complex 1 gamma-1 Large Chain)

AP1g1 (AP-1 Complex Subunit gamma-1, Adapter-related Protein Complex 1 Subunit gamma-1, Golgi Adaptor HA1/AP1 Adaptin Subunit gamma-1, Clathrin Assembly Protein Complex 1 gamma-1 Large Chain)

Cat no: A2298-48B

Supplier: United States Biological
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Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans Golgi network to lysosomes. Together with medium and small subunits, adaptins form a heterotetrameric complex called an adaptor whose role is to promote the formation of clathrin-coated pits and vesicles and to recognise sorting signals within the cytosolic tails of transmembrane cargo molecules. Gamma-adaptin protein belongs to the adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:4000 Immunohistochemistry (Frozen, paraffin): 1:200-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2298-48B
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a lyophilized powder. Reconstitute in 100ul sterile ddH2O.
P type: Pab
Isotype: IgG
Purity: Serum
References: 1. Peyrard M, et al. Genomics 50:275-280(1998). ; 2. Takatsu H, et al. J. Biol. Chem. 273:24693-24700(1998). ; 3. Deneka M, et al. EMBO J. 22:2645-2657(2003). ; 4. Nogi T, et al. Nat. Struct. Biol. 9:527-531(2002). ; 5. Robinson M.S. J. Cell Biol. 111:2319-2326(1990). ; 6. Kent H.M, et al. Structure 10:1139-1148(2002). ; 7. Woods,I.G, et al. Genome Res. 10 (12), 1903-1914 (2000). ; 8. Woods,I.G, et al. Genome Res. 15 (9), 1307-1314 (2005). ; 9. Strausberg, R.L, et al. Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002). ; 10. Rost, M, et al. J. Biol. Chem. 281 (39), 29297-29308 (2006)
Additional info: Recognizes. human AP1g1. Species Crossreactivity: rat. Species Sequence Homology: mouse, canine, zebrafish

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