AP1g1 (AP-1 Complex Subunit gamma-1, Adapter-related Protein Complex 1 Subunit gamma-1, Golgi Adaptor HA1/AP1 Adaptin Subunit gamma-1, Clathrin Assembly Protein Complex 1 gamma-1 Large Chain)

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. SUBUNIT: Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma1/AP1G1 or gamma2/AP1G2 and beta1A/AP1B1 or beta1B/AP1B1), a medium adaptin (mu1A/AP1M1 or mu1B/AP1M2) and a small adaptin (sigma1A/AP1S1 or sigma1B/AP1S2 or sigma1C/AP1S3). \n\nCellular Localization:\nGolgi apparatus. Cytoplasmic vesicle; cytoplasmic vesicle membrane; peripheral membrane protein; cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. \n\nAlternative Products:\n2 named isoforms produced by alternative splicing. \n\nTissue Specificity:\nWidely expressed. DISEASE: Deletion of the AP1B1 gene may play a role in the tumorigenesis of meningiomas. Belongs to the adaptor complexes large subunit family.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2298-48C

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.

P Type

Pab

Purity

Serum

References

1. Peyrard M, et al. Genomics 36:112-117(1996). ; 2. Peyrard M, et al. Hum. Mol. Genet. 3:1393-1399(1994). ; 3. Sacksteder C.A, et al. Biochemistry 45:8009-8022(2006). ; 4. Guilbaud C, et al. Mamm. Genome 8:651-656(1997). ; 5. Kirchhausen T, et al. Proc. Natl. Acad. Sci. U.S.A. 86:2612-2616(1989).

Additional Info

This antibody has been shown to be specific for AP-1 complex subunit beta-1. Species Crossreactivity: Rat and human, other species have not yet tested.