AP2S1 (Adaptor-related Protein Complex 2 sigma 1 Subunit, AP2 Complex Subunit sigma 1, AP-2 Complex Subunit sigma 1, AP 17, AP17, AP17 delta, Clathrin Assembly Protein 2 Small Chain, Clathrin-associated/assembly/adaptor Protein Small 2, CLAPS2, Clathrin C

Adapter-related protein complex 2 sigma-1 subunit (AP2S1, AP17) is the component of the adaptor complexes which links clathrin to receptors in coated vesicles.It is the smallest polypeptide chain component of AP-2, a heterotetramer composed of two large adaptins (alpha-type subunit, AP2 alpha, and beta-type subunit, AP2 beta), a medium adaptin (mu-type subunit, AP2 mu1) and a small adaptin (sigma-type subunit, AP2S1).AP-2 is one of two major clathrin-associated protein complexes that interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration (1, 2). \n \nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Not recommended for use in Immunocytochemistry or Immunohistochemistry (paraffin sections). Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:200\nWestern Blot: 1:1000-1:5000. Expected to detect a band at ~17kD.\nImmunoprecipitation: 1:10\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control:\nFetal brain lysate, HeLa cell lysate \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A2298-51T

Size

100ul

Applications

FC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Kirchhausen T, et al. J Biol Chem. 266(17):11153-7, 1991 2. The UniProt Consortium, The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195 (2008)

Additional Info

Recognizes human AP2S1. Species Crossreactivity: mouse and rat.