Home  >  Products  >  Apaf-1 (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease-activating Factor 1, APAF 1, APAF1, APAF-1, CED4, DKFZp781B1145, KIAA0413)
Apaf-1 (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease-activating Factor 1, APAF 1, APAF1, APAF-1, CED4, DKFZp781B1145, KIAA0413)

Apaf-1 (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease-activating Factor 1, APAF 1, APAF1, APAF-1, CED4, DKFZp781B1145, KIAA0413)

Cat no: A2298-68D

Supplier: United States Biological
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Apaf-1, a 135kD member of NOD family proteins, is a mammalian homolog of C.elegans CED4. It consists of an N-terminal caspase recruitment domain (CARD), a central nucleotide-binding oligomerization domain (NOD) and multiple WD40 repeats at the C-terminus. Apaf-1 exists as an inactive protein in the cells and requires ATP for activation. It binds with cytochrome C and dATP and oligomerizes into a large apoptosome complex responsible for the activation of procaspase-9. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Apaf-1 is an essential downstream effector of p53-mediated apoptosis and is inactivated in metastatic melanomas. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:50-1:100 Optimal dilutions to be determined by the researcher. Positive Control: Testis Cellular Localization: Cytoplasmic Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A2298-68D
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry
Size: 500ul
Form: Supplied as a liquid in 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Serum
References: 1. Reidl et.al Nature 434; 926-933 (14 April, 2005). 2. Chu et.al JBC 276 (12) 9239-9245 (23 March, 2001). 3. Robles et.al Cancer Res 61; 6660-6664 (2001).
Additional info: Recognizes human Apaf 1. Species Crossreactivity: mouse and rat.

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