Apaf-1 is a cytosolic protein that rests in a latent state until bound by cytochrome c (Apaf-2). The resulting Apaf-1/cytochrome c complex associates with the zymogen form of caspase-9 (Apaf-3) in the presence of dATP or ATP, promoting the autocatalytic activation of caspase-9. This antibody will be useful in studies of the activation of caspases through Apaf-1 and also in metastatic melanomas where Apaf-1 is inactivated which leads to defects in the execution of apoptotic cell death.
Cellular Localization: Cytoplasmic
Positive Control: Testis
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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