Home  >  Products  >  Apaf-1, CT (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease-activating Factor 1, APAF 1, APAF1, APAF-1, CED4, DKFZp781B1145, KIAA0413)
Apaf-1, CT (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease-activating Factor 1, APAF 1, APAF1, APAF-1, CED4, DKFZp781B1145, KIAA0413)

Apaf-1, CT (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease-activating Factor 1, APAF 1, APAF1, APAF-1, CED4, DKFZp781B1145, KIAA0413)

Cat no: A2298-65L

Supplier: United States Biological
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Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from Human and mouse and designated Apaf1 (for apoptosis protease-activating factor. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in Human tissues. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 20ug/ml Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HeLa or human heart tissue lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A2298-65L
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein G affinity chromatography.
References: 1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13. 2. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37. 3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89. 4. Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc Natl Acad Sci
Additional info: Recognizes human Apaf 1. Species Crossreactivity: mouse and rat.

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