Home  >  Products  >  Apaf-1, CT (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease Activating Factor 1, APAF 1, APAF1, Apoptotic Protease Activating Factor, CED4, KIAA0413)
Apaf-1, CT (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease Activating Factor 1, APAF 1, APAF1, Apoptotic Protease Activating Factor, CED4, KIAA0413)

Apaf-1, CT (Apoptotic Peptidase Activating Factor 1, Apoptotic Protease Activating Factor 1, APAF 1, APAF1, Apoptotic Protease Activating Factor, CED4, KIAA0413)

Cat no: A2298-65F

Supplier: United States Biological
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Apaf-1, a 135kD member of NOD family proteins, is a mammalian homolog of C.elegans CED4. It consists of an N-terminal caspase recruitment domain (CARD), a central nucleotide-binding oligomerization domain (NOD) and multiple WD40 repeats at the C-terminus. Apaf-1 exists as an inactive protein in the cells and requires ATP for activation. It binds with cytochrome C and dATP and oligomerizes into a large apoptosome complex responsible for the activation of procaspase-9. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Apaf-1 is an essential downstream effector of p53-mediated apoptosis and is inactivated in metastatic melanomas. Applications: Suitable for use in Immunohistochemistry (paraffin) and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 20ug/ml Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2298-65F
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Zou H., et al (1997) Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90:405-13, 2. Cecconi F., et al (1998) Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 94:727-37, 3. Li P, et al (1997) Cytochrome c and dATP-dependent formation of Apaf1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91:479-89, 4. Hu Y, et al (1998) Bcl-XL interacts with Apaf1 and inhibits Apaf1-dependent caspase-9 activation. PNAS 95:4386-91.
Additional info: Recognizes human Apaf1 (Apoptosis activating factor 1), a ubiquitously expressed cytoplasmic protein. Species Crossreactivity: Rat, Mouse.

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