APAF1, NT (Apoptotic Protease-activating Factor 1, APAF-1, KIAA0413)

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf-1 (for apoptosis protease-activating factor 1). Apaf-1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 can also associate with caspase-4 and caspase-8. Apaf-1 is ubiquitously expressed in human tissues.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa whole cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2298-65R

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Zou, H. et al. Cell 90, 405 (1997). 2. Cecconi, F. et al. Cell 94, 727 (1998). 3. Li, P. et al. Cell 91, 479 (1997). 4. Hu, Y. et al. Proc. Natl. Acad. Sci. USA 95, 4386 (1998).

Additional Info

Recognizes human Apaf-1. Species Crossreactivity: mouse and rat.