APC2, CT (Adenomatous Polyposis Coli Protein 2, Adenomatous Polyposis Coli Protein-like, APC-like, APCL)

The adenomatous polyposis coli tumor suppressor protein homologue APC2 (formerly known as APCL) is involved in the p53/Bcl-2-linked pathway of cell-cycle progression and cell death. APC2 is also heavily involved in the regulation of beta-Catenin transcriptional activity and the formation of beta-Catenin-TCF complexes, and as thus, may also be required for Wnt transduction in all cells. Loss of function mutations in one of the two human APC genes result in improper activation of the Wnt signaling pathway, and are one of the first steps in the development of colon cancer, as well as other tumors. Current research also speculates that APC2 interacts with and may also regulate the cytoskeleton. APC2 bears significant sequence homology to the tumor suppressor APC, and is expressed strongly and specifically in the central nervous system. The expression pattern of APC2 indicates a specific role in proliferation and differentiation in the central nervous system. APC2 is also expressed in several non-neuronal tissues and cell lines including breast, colon and ovary. Overexpressed human APC2 localizes to the perinuclear region and to some microtubles in the cell body. Endogenus human APC2 is diffusely distributed in the cytoplasm and co-localizes with both the Golgi apparatus and actin filaments, suggesting that it could have a role in cell motility or adhesion through interaction with actin filaments. Applications: Suitable for use in Immunoprecipitation, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:2000-1:10,000 Western Blot: 1:500-1:1000 Immunohistochemistry (Formalin fixed paraffin embedded): 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.