APC6 (ANAPC6, Anaphase Promoting Complex 6, CDC16)

APC6 (anaphase-promoting complex subunit 6) is a member of the E3 enzyme family. This protein contains TPR repeats and has a molecular weight of approximately 72kD. The APC6 protein is located at the centrosome and mitotic spindle. This protein is required for core stability in the spindle checkpoint pathway. The APC6 protein functions with other members of the APC complex as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition and spindle elongation. The APC6 protein comprises one subunit of the anaphase promoting complex including APC1-8 and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1.\n\nSpecificity:\nSpecific for the phosphorylated form of APC6 at the T580 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein.\n \nPhosphorylated: ppThr580\n \nLocalization: Nuclear\n\nLimitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 12 months from date of receipt.

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SPECIFICATIONS

Catalog Number

A2298-72L

Size

100ug

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.01% Sodium Azide Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Kallio M et al. Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events. J Cell Biol 141:1393-406 (1998). Ollendorff V & Donoghue DJ The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex. J Biol Chem 272:32011-8 (1997).

Additional Info

Recognizes human Apc6.