APE (APEX, APEX1, APEX nuclease multifunctional DNA repair enzyme, APX, APE1, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, DNA apurinic/apyrimidinic exonuclease/lyase)

Alternate Name: APEX, APEX1, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APE1, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, apurinic/apyrimidinic (abasic) endonuclease, deoxyribonuclease (apurinic or apyrimidinic)\n\nDomain Information: Exonuclease III [DNA replication, recombination, and repair]\n\nGene Ontology Terms: DNA-(apurinic or apyrimidinic site) lyase activity; uracil DNA N-glycosylase activity; endodeoxyribonuclease activity; transcription co-repressor activity; 3'-5' exonuclease activity; endonuclease activity; DNA binding; transcription co-activator activity; base-excision repair; DNA repair; transcription from Pol II promoter; obsolete; nucleus\n\nApplications: \nSuitable for use in ELISA, Western Blotting. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:32000\nWestern blot: 0.2-1ug/ml. ~35kD band seen in Human epidermoid fibroblast A431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.6kDa according to NP_001632.2; NP_542379.1; NP_542380.1.. Staining (0.5ug/ml) of A431 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See A2298-74N-P. Antibody

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A2298-74N

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Demple B, Herman T, Chen DS. , Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes., Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11450-4.

Additional Info

Recognizes human APE. NP_001632.2; NP_542379.1 and NP_542380.1 are variants that represent the same protein.