APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5) (HRP)

Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500\nImmunohistochemistry: 2.5ug/ml\nOptimal dilutions to be determined by researcher.\n\nPositive Control:\nMouse wild-type ES cell lysate.\n\nStorage and Stability:\nStore at 2-8 degrees C. Do Not Freeze! Aliquots are stable for at least 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

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SPECIFICATIONS

Catalog Number

A2298-76H

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, NHP, Prc, Xen/Amph, ZF/Fish

Form

Supplied as a liquid in PBS, pH 7.4. Labeled with HRP.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Wang, Y., R. Singh, et al. (2008) J. Biol. Chem. 283:4766-4777. 2. Pyo, J., Nah, J., Kim, H., et al. (2008) Autophagy. April 4(3):315-321. 3. Cadwell K, Liu JY, Brown SL, et al. (2008) Nature 456(7219):259-263. 4. Mizushima, N., et al. (2001) J. Cell. Biol. 152:657-668.

Additional Info

Selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing aa1-79. The calpain cleaved form of ATG5 is missing aa195-275. Cellular Localization: Cytoplasm. Co-localizes with non-muscle actin. Species Crossreactivity: human, mouse, rat, bovine, porcine, primate, Xenopus and Zebrafish. MW of human antigen: 33kD.