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APG5 (Autophagy Protein 5, ATG5, hAPG5, APG5-like, APG5L, Apoptosis-specific Protein, ASP, Autophagy Related 5)

Cat no: A2298-76K


Supplier: United States Biological
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APG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. APG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A2298-76K
Reactivities: Human
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
P type: Mab
Isotype: IgG2b,k
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes the 50kD human APG5 protein.

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