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APIP (Probable Methylthioribulose-1-phosphate Dehydratase, MTRu-1-P Dehydratase, APAF1-interacting Protein, CGI-29)

APIP (Probable Methylthioribulose-1-phosphate Dehydratase, MTRu-1-P Dehydratase, APAF1-interacting Protein, CGI-29)

Cat no: A2298-79H3

Supplier: United States Biological
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The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis3. Recently, Cho et al (4) have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204aa. An isoform of APIP, APIP2 encodes a 242aa protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Recombinant protein Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A2298-79H3
Reactivities: Human
Hosts: Mouse
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein G affinity chromatography.
References: 1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997; 90:405-13 2. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998; 94:727-37 3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation ofApaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89 4. Cho D-H, Hong Y-M, Lee H-J, Woo H-N, Pyo J-O, Mak TW, Jung Y-K. Induced Inhibition of Ischemic/Hypoxic Injury by APIP, a Novel Apaf-1-interacting protein. J Biol Chem, published on July 15, 2004 as Manuscript M405747200.
Additional info: Recognizes human APIP.

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