Apolipoprotein A-I (Apo A-I)

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). The apo-lipoprotein used here is derived from human plasma by density gradient ultracentrifugation and HPLC.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000- 1:10000\nImmunohistochemistry: Paraffin sections\nWestern Blot: 1:500- 1:1000.\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Secreted\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2299-08V

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Mouse

Form

Supplied as a liquid in 0.15M sodium chloride, 0.02M potassium phosphate, pH 7, 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hasebe, A., Pennock, N. D., Mu, H.-H., Chan, F. V., Taylor, M. L., Cole, B. C. (2006) A Microbial TLR2 Agonist Imparts Macrophage-Activating Ability to Apolipoprotein A-1, 177, 4826-4832.\n2. Duong. P.T., et al. Characterization of nascent HDL particles microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J. Lipid Res. 47:832-843, 2006

Additional Info

This antibody reacts with mouse apoLipoprotein A-I and has negligible crossreactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA.

Alternative Names

APOA1/APOC3 fusion gene, Apolipoprotein A I precursor, Apolipoprotein of high density lipoprotein