Apolipoprotein A-I (Apolipoprotein AI, Apo AI, Apo A-I, ApoA-I, Apo-AI, Apolipoprotein A1, APOA1, MGC117399)

APOA1 promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally. Variation in the latter step, in addition to modifications leading to so-called isoforms, is responsible for some of the polymorphism observed. APOA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the C-III gene is transcribed convergently in relation to A-I. Defects in the apolipoprotein A-1 gene are associated with HDL deficiency and Tangier disease.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:20,000\nWestern Blot: 1:1000-1:2000\nImmunohistochemistry (Paraffin): 5ug/ml\nIImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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