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Apolipoprotein A-IV (APOA4, MGC142154, MGC142156)

Apolipoprotein A-IV (APOA4, MGC142154, MGC142156)

Cat no: A2299-36G

Supplier: United States Biological
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Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.01-0.03ug/ml. ~ 45kD band observed in Human Duodenum lysates (calculated MW of 45.4kD according to NP_000473.2). Optimal dilutions to be determined by the researcher. Positive Control: Human Duodenum lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A2299-36G
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Spaulding, H.L., et al., Am. J. Physiol. Cell Physiol. 290: C95-C103 (2006).
Additional info: Recognizes human Apolipoprotein A-IV.

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