Apolipoprotein B (Apo B)

Lipids (triglyceride, cholesterol, phospholipids, and cholesteryl esters) are insoluble in plasma. These lipids combine with apolipoproteins to form lipoproteins. This process solubilizes lipids in plasma. Lipoproteins provide a transport system for lipids. They also play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism.\nApolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Increased levels of apo B100 are associated with an increased risk of coronary artery disease. Apo B exists in human plasma as two isoforms: apo B48 and apo B100. Apo B100 is synthesized in the liver. It is required for the assembly of very low density lipoproteins (VLDL). \n\nApplications: \nSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nLiver, HepG2 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2299-37A

Size

1ml

Applications

IP, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in 0.05M Tris-HCl pH 7.5, 0.5M sodium chloride, 0.1% sodium azide.

P Type

Pab

Purity

Serum, delipidated and defibrinated

Isotype

IgG

References

1. Antisense oligonucleotide-induced alternative splicing of the APOB mRNA generates a novel isoform of APOB. Khoo, B., Roca, X., Chew, S.L. and Krainer, A.R. (2007) BMC molecular biology 8:3. 2. Inhibition of apolipoprotein B and triglyceride secretion in human hepatoma cells (HepG2). Haghpassand, M., Wilder, D. and Moberly, J.B. (1996) Journal of lipid research 37:1468-1480. 3. Tunicamycin induces ubiquitination and degradation of apolipoprotein B in HepG2 cells Liao, W. and Chan, L. (2001) Biochemical Journal, 353:493-501. 4. Proteasome- mediated degradation of apolipoprotein B targets both nascent peptides cotranslationally before translocation and full-length apolipoprotein B after translocation into the endoplasmic reticulum. Liao, W., et al. (1998) Journal of Biological Chemistry, 273:27225-27230.

Additional Info

Recognizes human Apolipoprotein B. Monospecific by IEP with neat antibody vs. 2X pooled human serum and neat pooled human plasma.