Apolipoprotein B (Apo B, ApoB, ApoB Protein, Apolipoprotein B-48, ApoB-48, Apolipoprotein B-100, ApoB-100, Apo B-100, FLDB, LDLCQ4)

Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Apo B exists in human plasma as two isoforms: apo B48 and apo B100. Apo B100 is the major physiological ligand for the LDL receptor. It contains 4536aa residues. Apo B100 is the full length protein. Its gene has been mapped on the short arm of chromosome 2, with an ~length of 43kb and 29 exons. Apo B100 is synthesised in the liver. It is required for the assembly of very low density lipoproteins (VLDL). It does not interchange between lipoprotein particles, as do the other lipoproteins. It is found in IDL and LDL particles after the removal of the apolipoproteins A, E and C. Apo B48 is present in chylomicrons and chylomicron remnants. It plays an essential role in the intestinal absorption of dietary fats. Apo B48 is synthesized in the small intestine. It comprises the N-terminal 48% of apo B100. Apo B48 is the degraded protein. Apo B48 lacks the LDL receptor-binding domain of Apo B100. It is produced due to post-transscriptional Apo B100 mRNA editing at codon 2153, which creates a stop codon in the intestine instead of a glutamine in the liver.\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1000\nImmunohistochemistry (Frozen): 1:10 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months after receipt at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2299-52J

Size

1ml

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from 10mM sodium phosphate, pH 8.0, 100mM sodium chloride, 0.1% mannitol, 1mM sodium azide. Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Purified by sodium sulfate and DEAE-cellulose chromatography.

Isotype

IgG1

Additional Info

Recognizes human Apolipoprotein B (Apo-B) from human plasma. Specific for fragment T2, the carboxyterminal thrombolytic peptide of human Apo-B. No crossreactions with other human plasma proteins were detected.

Alternative Names

Apolipoprotein B (including Ag(x) Antigen)