Apolipoprotein B (Apo B) (Biotin)

Lipids (triglyceride (TG), cholesterol (Chol), phospholipids (PL), cholesteryl esters (CE)) are insoluble in plasma. These lipids combine with apolipoproteins to form lipoproteins. This process solubilizes lipids in plasma. Lipoproteins provide a transport system for lipids. They also play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Increased levels of apo B100 are associated with an increased risk of coronary artery disease. Apo B exists in human plasma as two isoforms: apo B48 and apo B100. Apo B100 is the major physiological ligand for the LDL receptor. It contains 4536 amino acid residues. Apo B100 is the full length protein. Its gene has been mapped on the short arm of chromosome 2, with an approximate length of 43 kilobases and 29 exons. The LDL-binding domain of the molecule is proposed to be located between the aa3129-3532. Apo B100 is synthesized in the liver. It is required for the assembly of very low density lipoproteins (VLDL).\n\nApplications: \nSuitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:4000-1:20,000\nWestern Blot: 1:400-1:2000\nImmunohistochemistry(Paraffin):1:100-1:1000\nImmunoprecipitation: 1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2299-39A

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Goat

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. No preservative and no stabilizing protein added. Labeled with Biotin.

P Type

Pab1

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human apolipoprotein B. Assay by IEP resulted in a single precipitin arc against anti goat serum. Has negligible crossreactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins. Typically less than 1% crossreactivity against other types of apolipoprotein was detected by ELISA against puri?ed standards. Non-speci?c crossreaction of anti-apolipoprotein antibodies with other human serum proteins is negligible.