Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549kD and is mostly synthesized in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800mg/L.
Applications:
Suitable for use in Immunodiffusion, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.