Apolipoprotein B48/100 (Apo B48/100) (HRP)

Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG CoA reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Increased levels of apo B100 are associated with an increased risk of coronary artery disease. Apo B exists in human plasma as two isoforms: apo B48 and apo B100. Apo B100 is the major physiological ligand for the LDL receptor. It contains 4536aa residues. Apo B100 is the full length protein. Its gene has been mapped on the short arm of chromosome 2, with an ~length of 43kb and 29 exons. Apo B100 is synthesised in the liver. It is required for the assembly of very low density lipoproteins (VLDL). It does not interchange between lipoprotein particles, as do the other lipoproteins. It is found in IDL and LDL particles after the removal of the apolipoproteins A, E and C. Apo B48 is present in chylomicrons and chylomicron remnants. It plays an essential role in the intestinal absorption of dietary fats. Apo B48 is synthesized in the small intestine. It comprises the N-terminal 48% of apo B100. Apo B48 is the degraded protein. \n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilutions: \nELISA: 1:2500-1:10,000.\nWestern Blot: 1:2500-1:10,000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

A2299-55A

Size

1ml

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder from 50mM PBS, 0.1M sodium chloride, pH 7.4, 10mg/ml BSA, 0.01% Thimerosal. Labeled with horseradish peroxidase(HRP). Reconstitute with 1ml sterile ddH2O.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

.1.Ng, C.J., et al., (2006), Journal of Biological Chemistry, 281(40): 29491-29500 2. Herscovitz, H., et al., (2001),J. Lipid Res., 42: 51-59 3. Kitchens, R.L., et al., (2003), Journal of Lipid Research, 44:2339-2348 4.Lin, M.C.M., et al., (1995), Journal of Lipid Research, 36: 1073-1081 5.Reardon, C.A., et al., (2004), Journal of Lipid Research, 45: 1347-1354 6. Schneider, M., et al., (2005), Journal of Lipid Research, 46: 769-778

Additional Info

Recognizes human Apo B-48/100. No crossreactivity with human Apo AI, Apo AII, Apo CI, Apo CII, Apo CIII or Apo E.