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Apolipoprotein D (ApoD, Apo-D)

Apolipoprotein D (ApoD, Apo-D)

Cat no: A2299-72C

Supplier: United States Biological
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Apolipoprotein D is a glycoprotein involved with the plasma lipid transport system. It may act in cholesterol esterification and the transport of products in this reaction. It is found widely in tissues including axillary apocrine glands, adrenal cortex, corpus luteum, nerves, pituitary, testis, cerebellum and renal tubules. It has been reported to be present in malignant prostatic glands, but absent from normal glands. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 1:40-1:80 for 1 hour at RT Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: ZR75 cell line Immunohistochemistry: Testis, Granular staining pattern of apolipoprotein D-containing lipid droplets Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2299-72C
Reactivities: Human
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 1ml
Form: Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
P type: Mab
Isotype: IgG1
Purity: Supernatant
References: 1. Hall R E, Aspinall J O, Horsfall D J, et al.. Expression of the androgen receptor and an androgen- esponsive protein, apolipoprotein D, in human breast cancer. British Journal of Cancer. 74:1175-1180 (1996). 2. Aspinall J O, Bentel J M, Horsfall D J, et al.. Differential expression of apolipoprotein D and prostate specific antigen in benign and malignant prostate tissues.The Journal of Urology. 154:622-628 (1995). 3. Diez-Itza I, Vizoso F, Merino A M, et al.. Expression and prognostic sigificance of apolipoprotein D in breast cancer. American Journal of Pathology. 144(2):310-320 (1994). 4. Weech P K, Camato R, Milne R W, et al.. Apolipoprotein D and cross reacting human plasma apolipoproteins identified using monoclonal antibodies. The Journal of Biological Chemistry. 26 (17):7941-7951 (1986).
Additional info: Recognizes Apolipoprotein D. Species Crossreactivity: Human.

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