Apolipoprotein F (Apo F), also known as lipid transfer inhibitor protein (LTIP) based on its ability to inhibit lipid transfer between lipoproteins, is a protein component of several lipoprotein classes and a physiologic regulator of cholesteryl ester transfer protein (CETP) function. Apo F activity was reported to be localized to LDL, consistent with its greater inhibitory activity on this lipoprotein.Apo F activity is controlled by its reversible incorporation into an inactive complex which provides for short-term fine-tuning of lipoprotein remodeling mediated by CETP. ApoF overexpression reduces HDL cholesterol levels by increasing clearance of HDL-CE.ApoF may be an important determinant of HDL metabolism and reverse cholesterol transport.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.3-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
Human Liver lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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